Abstract

La versión en inglés del resumen, por si en español no quedó claro... :P

The aim of this study was to develop and evaluate an extender solution for short-term storage of Penaeus (Litopenaeus) vannamei sperm to be used in artificial insemination (AI).

The sperm survival percentage was evaluated assessing sperm membrane integrity for 3 different extenders, calcium free artificial sea water (ASW), Hank’s balanced solution with calcium (HBS2), and calcium free Hank’s solution (HBS1), obtaining better results with ASW.

Data were analyzed using mixed linear models and logistic models, showing that sperm survival percentage (standard error) using ASW was 91.6 (30), and did not differ from HBS1 79.6 (31.4) (P>0.05), but was different from HBS2 59.5 (30) (P<0.05). p="0.0065.">

To assess nauplii production using ASW extender, 2 experiments were performed. The first experiment compared two treatments: sperm from half spermatophore (1) diluted with ASW and (2) undiluted, to inseminate four females per male in both treatments. Results showed that diluted semen yielded more nauplii than undiluted semen, with a mean (standard deviation) of 11915 (630) vs. 21589 (1471), respectively (P=0.0086). Female success for nauplii production was 4.9 times larger for diluted than undiluted treatment with 82.9% success when ASW was used.

The second experiment compared 3 treatments: undiluted sperm mass from one spermatophore (MP), same as MP but pouring ASW on it as a humectant (E1), and half sperm mass diluted on ASW (D1),to be used in two different AI male:female ratios 1:2 (MP/E1), and 1:4 (D1). Marginal differences between treatments (MP, D1 y E1) were observed for nauplii yielded (P=0.0992), with a mean (standard deviation) of 5388 (102) for MP, 102 (129) for E1, and 21 (90) for D1.

Using half spermatophore diluted with ASW allowed us to increase the number of females inseminated per male. It was possible to obtain nauplii from four different females per male in almost 86% of the cases, with a mean (standard error) of 3.43 (0.76) fertilized females per male.